土壤中产脲酶细菌的分离及其在微生物砂浆制备中的应用

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发布时间：2020-01-06 00:00:00

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土壤中产脲酶细菌的分离及其在微生物砂浆制备中的应用

Urease Produced Strain Isolation from Soil and Its Application on Microbial Mortar Preparation

２０13年第8期

迟缓芽孢杆菌；微生物矿化；碳酸钙；脲酶

Bacillus lentus; Microbial mineralization; Calcium carbonate; Urease

２０13年第8期

1000-4637（2013）08-13-04

国家自然科学基金（50908122）

李萌1,2，程晓辉2，杨钻2，郭红仙2

1.北京工商大学理学院，100048；2. 清华大学土木工程系，北京 100084

李萌1,2，程晓辉2，杨钻2，郭红仙2

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摘要：从土壤中筛选培育出一株脲酶高产菌株，经16S rDNA鉴定为迟缓芽孢杆菌（Bacillus lentus）。利用该菌株制备微生物砂浆，所制备的砂浆单轴抗压强度为2.4MPa。对所产生的物质进行X射线衍射分析，结果表明所形成的物质为方解石和球霰石，每克砂柱中碳酸钙的含量为163.15mg/g。通过扫描电子显微镜观察发现砂粒之间有球状及六面体状碳酸钙结晶生成，粘结后的砂柱中仍存在大量孔隙。 Abstract: A high yield urease produced strain is isolated from soil. It is identified as Bacillus lentus by 16S rDNA sequences. Mortar is prepared using the strain, uniaxial compressive strength of it is 2.4 MPa. Analysis by XRD, the new material is calcite and vaterite. The yield of calcium carbonate in sand column is of 163.15 mg/g sand. Through SEM observation found hexahedral shaped calcium carbonate crystallization generated, and there are still a large number of pores in the sand column after bonding.

英文名 : Urease Produced Strain Isolation from Soil and Its Application on Microbial Mortar Preparation

刊期 : ２０13年第8期

关键词 : 迟缓芽孢杆菌；微生物矿化；碳酸钙；脲酶

Key words : Bacillus lentus; Microbial mineralization; Calcium carbonate; Urease

刊期 : ２０13年第8期

DOI :

文章编号 : 1000-4637（2013）08-13-04

基金项目 : 国家自然科学基金（50908122）

作者 : 李萌1,2，程晓辉2，杨钻2，郭红仙2

单位 : 1.北京工商大学理学院，100048；2. 清华大学土木工程系，北京 100084

李萌1,2，程晓辉2，杨钻2，郭红仙2

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引用本文

Abstract: A high yield urease produced strain is isolated from soil. It is identified as Bacillus lentus by 16S rDNA sequences. Mortar is prepared using the strain, uniaxial compressive strength of it is 2.4 MPa. Analysis by XRD, the new material is calcite and vaterite. The yield of calcium carbonate in sand column is of 163.15 mg/g sand. Through SEM observation found hexahedral shaped calcium carbonate crystallization generated, and there are still a large number of pores in the sand column after bonding.

关键词:

迟缓芽孢杆菌；微生物矿化；碳酸钙；脲酶

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[1] Ivanov, V., Chu, J. Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ [J]. Reviews in Environmental Science and Biotechnology, 2008, 7(2): 139-153.

[2] DeJong, J.T., Fritzges, M.B., Nusslein, K. Microbially induced cementation to control sand response to undrained shear [J]. Journal of Geotechnical and Geoenvironmental Engineering, 2006, 132 (11): 1381.

[3] Mitchell, J.K., Hon, M., and Santamarina, J. C. Biological Considerations in Geotechnical Engineering [J]. Journal of Geotechbical and Geoenviromental Engineering, 2005(10): 1222-1233.

[4] Whiffin, V.S., Van Paassen, L.A., Harkes, M.P.. Microbial carbonate precipitation as a soil improvement technique [J]. Geomicrobiology Journal, 2007, 24 (5): 417-423.

[5] Fujita, Y., Ferris, E.G., Lawson, R.D., Colwell, F. S., Smith, R.W. Calcium carbonate precipitation by ureolytic subsurface bacteria [J]. Geomicrobiology Journal, 2000(17): 305-318.

[6] 李沛豪, 屈文俊. 细菌诱导矿化保护历史建筑遗产的机理及效果[J]. 硅酸盐学报, 2009, 37(4): 497-505.

[7] Cheng L, Cord-Ruwisch R. In situ soil cementation with ureolytic bacteria by surface percolation [J]. Ecological Engineering, 2012(42): 64-72.

[8] Li M, Cheng XH, Guo HX. Heavy metal removal by biomineralization of urease producing bacteria isolated from soil [J]. Int Biodeterior Biodegr, 2013(76): 81-85.

[9] van Paassen, L.A., Whiffin, V.S., Harkes, M.P. Microbial carbonate precipitation as a soil improvement technique [J]. Geomicrobiology Journal, 2007(24): 417-423.

[10] Whiffin, V.S., Lambert, J.W.M., Van, R. Biogrout and biosealing-pore space engineering with bacteria [J]. Geostrata- Geo Institute for ASCE, 2005(5): 13-16.

[11] Yang, Z., Cheng XH. A performance study of high-strength microbial mortar produced by low pressure grouting for the reinforcement of deteriorated masonry structures [J]. Construction and Building Materials, 2013(41): 505–515.

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土壤中产脲酶细菌的分离及其在微生物砂浆制备中的应用

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